Locus: CC0277
Primer Set: 1

CC0277F (Forward)
GCAGCCAAGCGTTCATAGC
CC0277R (Reverse)
CCGATCGAAGGTAGCCAATG

PCR Condition
Anneal. temp.: 57
PCR cycle:  40
MgCl2: 2 mM

Product size: 400 bp

Restriction Enzyme: MspI 
PIC: 0.06