Locus: CC0592
Primer Set: 1

CC0592F (Forward)
ATTTGAGTTAGTTTGCGATGG
CC0592R (Reverse)
AGAAGATCCTCCTGCGTAAT

PCR Condition
Anneal. temp.: 57
PCR cycle:  40
MgCl2: 2 mM

Product size: >1500 bp

Restriction Enzyme: RsaI
PIC: 0.5

Restriction Enzyme: AluI
PIC: 0.5

Restriction Enzyme: MboII
PIC: 0.5

Restriction Enzyme: HinfI
PIC: 0.5

Restriction Enzyme: DdeI
PIC: 0.5

Restriction Enzyme: NciI
PIC: 0.5

Restriction Enzyme: Bst0I
PIC: 0.5