Locus: CC0831
Primer Set: 1

CC0831F (Forward)
GCTCGTGCATCAAGGA
CC0831R (Reverse)
TGGGTTAAAAATAACTGGACA

PCR Condition
Anneal. temp.: 54
PCR cycle:  40
MgCl2: 2 mM

Product size: >1500 bp

Restriction Enzyme: RsaI
PIC: 0.35

Restriction Enzyme: SspI
PIC: 0.4

Restriction Enzyme: NdeII
PIC: 0.33