Locus: CC2448
Primer Set: 1

CC2448F (Forward)
ATCCTAAGTCCCCAGAAAGT
CC2448R (Reverse)
GAATTGGAATGGCATAAAGA

PCR Condition
Anneal. temp.: 57
PCR cycle:  40
MgCl2: 2 mM

Product size: >1500 bp

Restriction Enzyme: RsaI
PIC: 0.34

Restriction Enzyme: HaeIII
PIC: 0.34

Restriction Enzyme: TaqI
PIC: 0.26