Locus: CC3162
Primer Set: 1

CC3162F (Forward)
ATGGAGTCGCCACAAAAGAC
CC3162R (Reverse)
GTCTCGCATCAAATCTCCGT

PCR Condition
Anneal. temp.: 60
PCR cycle:  40
MgCl2: 2 mM

Product size: 700 bp

Restriction Enzyme: HhaI
PIC: 0.26