Locus: CC3455
Primer Set: 1

CC3455F (Forward)
TAACAAGGGAAAACACAGCA
CC3455R (Reverse)
CCGCATCCCATTGAACAGCA

PCR Condition
Anneal. temp.: 60
PCR cycle:  40
MgCl2: 2 mM

Product size: 1000 bp

Restriction Enzyme: RsaI
PIC: 0.36

Restriction Enzyme: HinfI
PIC: 0.28