Locus: CC3816
Primer Set: 1

CC3816F (Forward)
AGTCAGAGCTGCCTGGAAAG
CC3816R (Reverse)
GCCACGAAGGGATTCATTTA

PCR Condition
Anneal. temp.: 60
PCR cycle:  40
MgCl2: 2 mM

Product size: >1500 bp

Restriction Enzyme: RsaI
PIC: 0.32

Restriction Enzyme: DdeI
PIC: 0.06