Locus: CC3861
Primer Set: 1

CC3861F (Forward)
CTGACACTGATGAGGCCAGA
CC3861R (Reverse)
TGCAAAGATGGAAAACACCA

PCR Condition
Anneal. temp.: 57
PCR cycle:  40
MgCl2: 2 mM

Product size: 800 bp

Restriction Enzyme: MspI
PIC: 0.37

Restriction Enzyme: RsaI
PIC: 0.37

Restriction Enzyme: AluI
PIC: 0.36

Restriction Enzyme: HaeIII
PIC: 0.35

Restriction Enzyme:  DdeI
PIC: 0.36

Restriction Enzyme: BamHI
PIC: 0.36

Restriction Enzyme: DdeI
PIC: 0.36

Restriction Enzyme: NdeII
PIC: 0.26