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Artificial Seeds Enveloped with Dialysis Membrane Germinate Quickly under Non-aseptic Conditions

KINOSHITA Isao


Abstract
Previously, we had found that double layered artificial seeds of Japanese white birch whose inner layer contained a large quantity of sucrose had germinated in non-sterilized perlite. However, this type of artificial seed did not germinate on other types of substrates, such as vermiculite. To prevent the diffusion of sucrose from artificial seeds to non-sterilized substrate, artificial seeds were enveloped in a dialysis membrane. The enveloped artificial seeds germinated quickly in non-sterilized vermiculite.

Key words : Artificial seed, Dialysis membrane, Japanese white birch, Non-sterilized vermiculite

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